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Separation and identification of flavones and flavone glycosides
Summary
Type:
TLC
Application #:
402040
Detection:
Spray methanolic solution of 1% diphenylboric acid and 5% polyethylene glycol
Conditions:
Eluents 1: Methanol-water-acetic acid 25:25:3
Eluents 2: Methanol-water-acetic acid 78:25:3
Authors:
Pieroni A
Source:
J Planar Chromat 11 (1998) 230-232
Details
Legend: Compound Rf (eluent 1) Rf (eluent 2) Colour after spray reagent Luteolin-4-glucoside 0.32 0.55 yellow-green Apigenin-7-O-glucoside 0.38 0.68 green Luteolin-7-O-glucoside 0.43 0.77 yello
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